CD BioSciences is a US-based biotechnology firm that focuses on developing imaging technology. Recently, CD BioSciences announced the launch label-free live-cell imaging services to scientific studies. These include measuring and counting cell growth, proliferation, and cell count without staining.
- (1888PressRelease), January 6, 2023 – Cell research is a major part of biological research. Imaging live cells, whether viewed through a microscope or with the naked eye, has been a key part of biological research. Fluorescence microscopy is a highly sensitive, specific and precise imaging method for living cells. Because there are a limited number of fluorescent chemicals in living cells, fluorescence-labeled antibodies and probes can pinpoint the exact location of certain molecules. Fluorescence imaging has a few drawbacks. For example, fluorescent labels can be difficult to read and predict complex biological systems. A cell imaging device that is ideal for research in cells should be sensitive and specific, but not require labeling.
Live-cell imaging that is label-free does not require the use of any exogenous chemicals to interfere with the study’s physiological response. It also doesn’t need fixatives which could cause cell death. Scientists have used this imaging method to understand cell dynamics and function. CD BioSciences offers comprehensive services that are based on live cell imaging technology.
Microscopy using phase contrast (PC), differential interference contrast, and DIC microscopy is one of the most widely used label-free imaging methods in biological research. These techniques use specific optics that convert the phase differences of cells or intracellular property changes into light waves with different amplitudes. Human eyes can detect changes in light waves, but not phase variations. These changes are recorded on cameras as changes to pixel intensity. Low image contrast and intensity can hinder automatic segmentation at high cell density.
Quantitative phase imaging does not require labeling and has a higher contrast and content than other techniques. The generation of single-cell metrics using high-contrast images is possible through better segmentation and tracking. This results in unlabeled dynamic Phenotypes which describe temporal and cell-to-cell heterogeneity. Single-cell dynamic phenotypes can be more useful in clinical applications, like cancer therapy, as they are more likely to differentiate the cells’ response to treatment from those at the population level. The data obtained can also be used to expand or analyze the existing cells, maximising the benefits of personalized and regenerative medicine.
For customers interested in more information about label-free live cell imaging solutions or other technologies, please visit CD BioSciences at https://www.bioimagingtech.com.
Information about CD BioSciences
CD BioSciences has been a pioneer in the field of biotechnology and imaging technology development for over ten years. The scientists at CD BioSciences can use high-content imaging and nanoparticle imaging as well as imaging flow cytometry and time-lapse imaging to capture cell structure and cell movement. They also have the ability to identify pathogen infections mechanisms, interactions among protein molecules, and cell structures. The team can also use powerful analytical software to take images of cells, tissues, and subcellular structures to gain useful information.